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MM Enot, F Weiland, P Mittal, P Hoffmann, M Sillero-Mahinay and T Pukala
Differential proteome analysis of the leaves of lead hyperaccumulator, Rhoeo discolor (L. Her.) Hance
Journal of Mass Spectrometry,  e4689 (accepted 20/11/2020)

B Jovcevski, S Das, S Smid and TL Pukala
Polyphenol honokiol and flavone 2′,3′,4′-trihydroxyflavone differentially interact with α-synuclein at distinct phases of aggregation
Chemical Neuroscience, 2020, (in press, accepted 13/11/2020)


S Nguyen, B Jovcevski, TL Pukala and JB Bruning
Nucleoside selectivity of Aspergillus fumigatus nucleoside-diphosphate kinase
The FEBS Journal, 2020, (in press, accepted 15/10/2020)

KG Stevens and TL Pukala
Conjugating immunoassays to mass spectrometry: Solutions to contemporary challenges in clinical diagnostics
Trends in Analytical Chemistry, 2020, 132:116064


M Salman Sajid, B Jovcevski, P Mittal, B Fatima, D Hussain, F Jabeen, M Naeem Ashiq, TL Pukala and M Najam-ul-Haq
Glycosylation heterogeneity and low abundant serum glycoproteins MS analysis by boronic acid immobilized Fe3O4@1,2-Epoxy-5-Hexene/DVB magnetic core shell nanoparticles
Microchemical Journal, 2020, 159:105351


LM Sternicki, S Nguyen, KJ Pacholarz, P Barran, NR Pendini, GW Booker, Y Huet, R Baltz, KL Wegener, TL Pukala and SW Polyak

Biochemical characterisation of class III biotin protein ligases from Botrytis cinerea and Zymoseptoria tritici
Archives of Biochemistry and Biophysics, 2020, 691:108509


JR Horsley, B Jovcevski, KL Wegener, J Yu, TL Pukala and AD Abell
Rationally designed peptide-based inhibitor of Aβ42 fibril formation and toxicity: a potential therapeutic strategy for Alzheimer's disease
Biochemical Journal, 2020, 477(11):2039-2054

MS Sajid, B Jovcevski, TL Pukala, F Jabeen and M Najam-ul-Haq
Fabrication of piperazine functionalized polymeric monolithic tip for rapid enrichment of glycopeptides/glycans
Analytical Chemistry, 2020, 92(1):683-689

HM Sanders, B Jovcevski, JA Carver and TL Pukala
The molecular chaperone β-casein prevents amorphous and fibrillar aggregation of α-lactalbumin by stabilisation of dynamic disorder
Biochemical Journal, 2020, 477(3):629-643

CR Wang, ER Bubner, B Jovcevski, P Mittal and TL Pukala
Interrogating the higher order structures of snake venom proteins using an integrated mass spectrometric approach
J Proteomics, 2020, 216:103680

AJ Hayes, J Satiaputra, LM Sternicki, AS Paparella, Z Feng, KJ Lee, BB Rodriguez, W Tieu, BA Eijkelkamp, KE Shearwin, TL Pukala, AD Abell, GW Booker and SW Polyak
Advanced resistance studies identify two discrete mechanisms in staphylococcus aureus to overcome antibacterial compounds that target biotin protein ligase
Antibiotics, 2020, 9(4):1-20


TN Aung, S Nourmohammadi, Z Qu, Y Harata-Lee, J Cui, HY Shen, AJ Yool, T Pukala, H Du, RD Kortschak, W Wei and DL Adelson
Fractional deletion of compound kushen injection indicates cytokine signaling pathways are critical for its perturbation of the cell cycle

Scientific Reports, 2019, 9(1):14200


B Jovcevski and TL Pukala

Mass spectrometry and its applications

In V. Ramesh (Ed.), Biomolecular and Bioanalytical Techniques Theory, Methodology and Applications (pp. 219-253). Wiley.

C Iacobucci, C Piotrowski, R Aebersold, BC Amaral, P Andrews, K Bernfur, C Borchers, NI Brodie, JE Bruce, Y Cao, S Chaignepain, JD Chavez, S Claverol, J Cox, T Davis, G Degliesposti, M-Q Dong, N Edinger, C Emanuelsson, M Gay, M Götze, F Gomes-Neto, FC Gozzo, C Gutierrez, C Haupt, AJR Heck, F Herzog, L Huang, MR Hoopmann, N Kalisman, O Klykov, Z Kukačka, F Liu, MJ MacCoss, K Mechtler, R Mesika, RL Moritz, N Nagaraj, V Nesati, AGC Neves-Ferreira, R Ninnis, P Novák, FJ O’Reilly, M Pelzing, E Petrotchenko, L Piersimoni, M Plasencia, T Pukala, KD Rand, J Rappsilber, D Reichmann, C Sailer, CP Sarnowski, RA Scheltema, C Schmidt, DC Schriemer, Y Shi, JM Skehel, M Slavin, F Sobott, V Solis-Mezarino, H Stephanowitz, F Stengel, CE Stieger, E Trabjerg, M Trnka, M Vilaseca, R Viner, Y Xiang, S Yilmaz, A Zelter, D Ziemianowicz, A Leitner and A Sinz
First community-wide, comparative cross-linking mass spectrometry study
Analytical Chemistry, 2019, 91(11):6953-6961


LJ Alcock, BL Oliveira, MJ Deery, TL Pukala, MV Perkins, GJL Bernardes and JM Chalker
Norbornene probes for the detection of cysteine sulfenic acid in cells
ACS Chemical Biology, 2019, 14(4):594-598

J Satiaputra, LM Sternicki, AJ Hayes, TL Pukala, GW Booker, KE Shearwin and SW Polyak
Native mass spectrometry identifies an alternative DNA-binding pathway for BirA from Staphylococcus aureus
Scientific Reports, 2019, 9

Y Liu, B Jovcevski and TL Pukala
C-Phycocyanin from spirulina inhibits alpha-synuclein and amyloid-beta fibril formation but not amorphous aggregation
Journal of Natural Products, 2019, 82(1):66-73

Full text available here

J Li, A Begbie, BJ Boehm, A Button, C Whidborne, Y Pouferis, DM Huang and TL Pukala
Ion mobility-mass spectrometry reveals details of formation and structure for GAA•TCC DNA and RNA triplexes
Journal of the American Society for Mass Spectrometry, 2019, 30(1):103-112


T Pukala
Importance of collision cross section measurements by ion mobility-mass spectrometry in structural biology

Rapid Communications in Mass Spectrometry, 2019, 33(S3): 72– 82
Full text available here

JA Harrison, C Kelso, TL Pukala and JL Beck
Conditions for Analysis of Native Protein Structures Using Uniform Field Drift Tube Ion Mobility Mass Spectrometry and Characterization of Stable Calibrants for TWIM-MS

Journal of the American Society for Mass Spectrometry, 2019, 30(2):256-267


TR Young, TL Pukala, R Cappai, AG Wedd and Z Xiao
The human amyloid precursor protein binds copper ions dominated by a picomolar-affinity site in the helix-rich E2 domain
Biochemistry, 2018, 57(28):4165-4176

RL Frkic, AC Marshall, A-L Blayo, TL Pukala, TM Kamenecka, PR Griffin and JB Bruning
PPARγ in complex with an antagonist and inverse agonist: a tumble and trap mechanism of the activation helix
iScience, 2018, 5:69-79

S Das, TL Pukala and SD Smid
Exploring the structural diversity in inhibitors of α-synuclein amyloidogenic folding, aggregation, and neurotoxicity
Frontiers in Chemistry, 2018, 6:181

SA Child, Justin M Bradley, TL Pukala, DA Svistunenko, NE Le Brun and SG Bell
Electron transfer ferredoxins with unusual cluster binding motifs support secondary metabolism in many bacteria
Chemical Science, 2018,

BJ Boehm, C Whidborne, AL Button, TL Pukala and DM Huang
DNA triplex structure, thermodynamics, and destabilisation: insight from molecular simulations
Physical Chemistry Chemical Physics, 2018, 20(20):14013-14023

LJ Alcock, KD Farrell, MT Akol, GH Jones, MM Tierney, HB Kramer, TL Pukala, GJL Bernardes, MV Perkins and JM Chalker
Norbornene probes for the study of cysteine oxidation
Tetrahedron, 2018, 74(12):1220-1228


S Das, L Stark, IF Musgrave, T Pukala and SD Smid
Bioactive polyphenol interactions with beta amyloid: a comparison of binding modelling, effects on fibril and aggregate formation and neuroprotective capacity
Food & Function, 2016, 7(2):1138-1146

AN Calabrese, Y Liu, T Wang, IF Musgrave, TL Pukala, RF Tabor, LL Martin, JA Carver and JH Bowie
The amyloid fibril-forming properties of the amphibian antimicrobial peptide uperin 3.5
ChemBioChem, 2016, 17(3):239-246


Y Liu, M Graetz, L Ho and TL Pukala
Ion mobility-mass spectrometry-based screening for inhibition of α-synuclein aggregation
European Journal of Mass Spectrometry, 2015, 21(3):255-264

AN Calabrese, JH Bowie and TL Pukala
Structural analysis of calmodulin binding by nNOS inhibitory amphibian peptides
Biochemistry, 2015, 54(2):567-576


Y Liu, JA Carver, LH Ho, AK Elias, IF Musgrave and TL Pukala
Hemin as a generic and potent protein misfolding inhibitor

Biochemical and Biophysical Research Communications, 2014, 454(2):295-300

Y Liu, J Carver, A Calabrese and T Pukala
Gallic acid interacts with α-synuclein to prevent the structural collapse necessary for its aggregation
Biochimica et Biophysica Acta, 2014, 1844(9):1481-1485


DM Williams and TL Pukala
Novel insights into protein misfolding diseases revealed by ion mobility-mass spectrometry
Mass Spectrometry Reviews, 2013, 369-87(32):169-187

DE Scott, MT Ehebauer, T Pukala, M Marsh, TL Blundell, AR Venkitaraman, C Abell and M Hyvonen
Using a fragment-based approach to target protein-protein interactions
ChemBioChem, 2013, 14(3):332-342

T Pukala
Mass spectrometry: mapping large stable protein complexes
In Encyclopedia of Biophysics (G Roberts, Ed), 2013, pp 1422-1429, Springer, Germany

Y Liu, T Pukala, I Musgrave, D Williams, F Dehle and J Carver
Gallic acid is the major component of grape seed extract that inhibits amyloid fibril formation
Bioorganic & Medicinal Chemistry Letters, 2013, 23(23):6336-6340

J Cottam, D Scanlon, J Karas, A Calabrese, T Pukala, B Forbes, J Wallace and A Abell
Chemical synthesis of a fluorescent IGF-II analogue
International Journal of Peptide Research and Therapeutics, 2013, 19(1):61-69

AN Calabrese, T Wang, JH Bowie and TL Pukala
Negative ion fragmentations of disulfide-containing cross-linking reagents are competitive with aspartic acid side-chain-induced cleavages
Rapid Communications in Mass Spectrometry, 2013, 27(1):238-248

A Calabrese and T Pukala
Chemical cross-linking and mass spectrometry for the structural analysis of protein assemblies
Australian Journal of Chemistry, 2013, 66(7):749-759



G McColl, BR Roberts, TL Pukala, VB Kenche, CM Roberts, CD Link, TM Ryan, CL Masters, KJ Barnham, AI Bush and RA Cherny
Utility of an improved model of amyloid-beta (Abeta 1-42) toxicity in Caenorhabditis elegans for drug screening for Alzheimer's disease

Molecular Neurodegeneration, 2012, 7:57

MHM Leung, P Mohan, TL Pukala, DB Scanlon, SF Lincoln and TW Kee
Reduction of copper(ii) to copper(i) in the copper-curcumin complex induces decomposition of curcumin

Australian Journal of Chemistry, 2012, 65(5):490-495

AN Calabrese, LA Speechley and TL Pukala
Characterisation of calmodulin structural transitions by ion mobility mass spectrometry

Australian Journal of Chemistry, 2012, 65(5):504-511

AN Calabrese, NJ Good, T Wang, J He, JH Bowie and TL Pukala
A negative ion mass spectrometry approach to identify cross-linked peptides utilizing characteristic disulfide fragmentations
Journal of the American Society for Mass Spectrometry, 2012, 23(8):1364-1375


T Wang, HJ Andreazza, TL Pukala, PJ Sherman, AN Calabrese and JH Bowie
Histidine-containing host-defence skin peptides of anurans bind Cu2+. An electrospray ionisation mass spectrometry and computational modelling study

Rapid Communications in Mass Spectrometry, 2011, 25(9):1209-1221

L Sanchez, S Madurga, T Pukala, M Vilaseca, C Lopez-Iglesias, CV Robinson, E Giralt and N Carulla
Aβ40 and Aβ42 amyloid fibrils exhibit distinct molecular recycling properties
Journal of the American Chemical Society, 2011, 133(17):6505-6508

TL Pukala
Mass spectrometry for structural biology: determining the composition and architecture of protein complexes
Australian Journal of Chemistry, 2011, 64(6):681-691

Y Liu, LH Ho, JA Carver and TL Pukala
Ion mobility mass spectrometry studies of the inhibition of alpha synuclein amyloid fibril formation by (–)-epigallocatechin-3-gallate

Australian Journal of Chemistry, 2011, 64(1):36-40

RJ Jackway, TL Pukala, SC Donnellan, PJ Sherman, MJ Tyler and JH Bowie
Skin peptide and cDNA profiling of Australian anurans: genus and species identification and evolutionary trends

Peptides, 2011, 32(1):161-172


TL Pukala, BT Ruotolo, M Zhou, A Politis, R Stefanescu, JA Leary and CV Robinson
Subunit architecture of multiprotein assemblies determined using restraints from gas-phase measurements
Structure, 2009, 17(9):1235-1243

JR Doyle, JH Bowie, RJ Jackway, LE Llewellyn, TL Pukala, MA Apponyi and GW Booker
Anuran host-defense peptides that complex with Ca2+ calmodulin and inhibit the synthesis of the cell signaling agent nitric oxide by neuronal nitric oxide synthase
In Bioactive Peptides (J Howl, and S Jones, Eds), 2009, pp 315-332, Taylor and Frances Publishing Group, UK


TL Pukala, T Urathamakul, SJ Watt, JL Beck, RJ Jackway and JH Bowie
Binding studies of nNOS-active amphibian peptides and Ca2+ calmodulin, using negative ion electrospray ionisation mass spectrometry
Rapid Communications in Mass Spectrometry, 2008, 22(22):3501-3509

RJ Jackway, TL Pukala, VM Maselli, IF Musgrave, JH Bowie, Y Liu, KH Surinya-Johnson, SC Donnellan, JR Doyle, LE Llewellyn and MJ Tyler
Disulfide-containing peptides from the glandular skin secretions of froglets of the genus Crinia: structure, activity and evolutionary trends
Regulatory Peptides, 2008, 151(1-3):80-87

JD Gehman, F Luc, K Hall, TH Lee, MP Boland, TL Pukala, JH Bowie, MI Aguilar and F Separovic
Effect of antimicrobial peptides from Australian tree frogs on anionic phospholipid membranes
Biochemistry, 2008, 47(33):8557-8565


TL Pukala, JR Doyle, LE Llewellyn, L Kuhn-Nentwig, MA Apponyi, F Separovic and JH Bowie
Cupiennin 1a, an antimicrobial peptide from the venom of the neotropical wandering spider Cupiennius salei, also inhibits the formation of nitric oxide by neuronal nitric oxide synthase
Febs Journal, 2007, 274(7):1778-1784

TL Pukala, MP Boland, JD Gehman, L Kuhn-Nentwig, F Separovic and JH Bowie
Solution structure and interaction of cupiennin 1a, a spider venom peptide, with phospholipid bilayers
Biochemistry, 2007, 46(11):3576-3585


TL Pukala, JH Bowie, VM Maselli, IF Musgrave and MJ Tyler
Host-defence peptides from the glandular secretions of amphibians: structure and activity
Natural Product Reports, 2006, 23(3):368-393

TL Pukala, T Bertozzi, SC Donnellan, JH Bowie, KH Surinya-Johnson, Y Liu, RJ Jackway, JR Doyle, LE Llewellyn and MJ Tyler
Host-defence peptide profiles of the skin secretions of interspecific hybrid tree frogs and their parents, female Litoria splendida and male Litoria caerulea
Febs Journal, 2006, 273(15):3511-3519


TL Pukala, CS Brinkworth, JA Carver and JH Bowie
Investigating the importance of the flexible hinge in caerin 1.1: solution structures and activity of two synthetically modified caerin peptides

Biochemistry, 2004, 43(4):937-944

CS Brinkworth, TL Pukala, JH Bowie and MJ Tyler
Host defence peptides from the skin glands of Australian amphibians. Caerulein neuropeptides and antimicrobial, anticancer, and nNOS inhibiting citropins from the Glandular Frog Litoria subglandulosa

Australian Journal of Chemistry, 2004, 57(7):693-701

M Apponyi, T Pukala, C Brinkworth, V Maselli, J Bowie, M Tyler, G Booker, J Wallace, J Carver, F Separovic, J Doyle and L Llewellyn
Host-defence peptides of Australian anurans: structure, mechanism of action and evolutionary significance
Peptides, 2004, 25(6):1035-1054


2004 - 2010
2010 - 2020
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